ABSTRACT
Background: It is a continuing effort to develop new anticancer drugs. It has been reported that albendazole has antiproliferative effects on several tumor cell lines. The aim of this study was to evaluate the killing effect of albendazole on HT-29 human colorectal cancer cells
Materials and methods: Cultured HT-29 cells were treated with albendazole in 0.1, 1 and 10 microgram/ml concentrations for 1, 3 and 5 days. Cellular viability and proliferation were evaluated by MTT and Trypan blue assays at determined days after treatment
Results: The comparison of cells count and viability in control group and treated groups with albendazole at different concentrations showed significant differences in days 1, 3 and 5
Conclusion: Albendazole can suppress HT-29 cells proliferation and induces cell death in a dose and time dependent manner
ABSTRACT
Background: Glioblastoma [U-87] is the most common and most malignant of the glial tumors that appeared in the central nervous system. This is the first study that has evaluated the cytotoxic effects of various Girard-P reagent-based Schiff bases and their dimethyltin [IV] complexes on Glioblastoma cell line, U-87, viability
Materials and methods: In this experimental study, U-87 cell line was grown in DMEM supplemented with 10% FBS, penicillin/streptomycin [100 U/ml, 100 micro.g/ml] at 37 °C in 5% CO2, then the effects of different concentrations of complexes on Glioblastoma cell were evaluated by MTT and DAPI staining
Results: Herein, we demonstrated that these compounds had cytotoxicity effects of Glioblastoma cancer cells. In a dose dependent manner, a significant cytotoxicity was observed with increasing of PlMandP3
Conclusion: The results showed that Girard-P reagent-based Schiff bases and their dimethyltin [IV] complexes have the ability to induce cytotoxicity in the Glioblastoma cancer cell line in lower mg/ml concentrations. In conclusion, these findings may introduce a new view on the mode of action and possible application of new compounds in the cancer chemotherapy
Subject(s)
Schiff Bases , In Vitro Techniques , Neoplasms , Cell Line , Organotin Compounds , Antineoplastic AgentsABSTRACT
Background: Human induced pluripotent cells [hiPSCs] could be established as promising new resources for obtaining differentiated cells for cell therapy. iPSCs have the potential to use as multipurpose research and clinical tools to understand model diseases, develop and screen candidate drugs, and deliver cell-replacement therapy to support regenerative medicine. Here, we demonstrated the ability of human iPSCs to differentiate into adipocyte and osteoblast fate
Materials and methods: In this experimental study, human iPSCs were culture-expanded. HiPSCs were then cultivated in the osteogenic and adipogenic conditions for 21 days after which differentiations were evaluated by specific staining as well as qRT-PCR analysis for related gene expression
Results: In osteoinductive cultures, the cells formed nodules which were positively stained red following alizarin red staining. In adipogenic cultures, the cells developed some lipid droplets which were positively stained red with oil red. According to qRT-PCR analysis, the bone-related genes including osteocalcin and osteopontin, and also the adipocyte-specific genes such as PPAR and LPL were expressed in the osteogenic and adipogenic cultures, respectively
Conclusion: Taken together, hiPSCs are pluripotent cells with the ability to differentiate into osteocytic and adipocytic cell lineages
ABSTRACT
The rat pheochromocytoma cell line [PC 12] differentiates and converts into neuron-like cells in in vitro condition under inductive factors. Researchers have shown that different growth factors, like neurotrophic giowth factor [NGF] and basic fibroblast growth factor [bFGF], have different effects in proliferation, survival and differentiation of the cells. It was hypothesized that porous biodegradable polymer scaffolds support the formation of complex 3D tissues during differentiation of PC12 cells. The aim of this study was to evaluate the role of nanofibrous scaffold PCL/gelatin on neuronal differentiation of PC 12 cells. In this basic study the PC12 cells were seeded on PCL/gelatin under identical media and growth factor supplementation conditions. Gene expression including Nestin and Map2 [Microtubule-associated protein 2] was analyzed using quantitative RT-PCR and immunostaining. Cellular morphology was analyzed with light microscopy; sphere ultra structure was analyzed with scanning electron microscopy. PC 12 cells could efficiently differentiate into neuron-like cells on 3D culture and PCL/gelatin scaffold had no adverse effect and toxicity on PC 12 cells. Using tissue engineering provides a potential mechanism for creating viable human neural tissue structures for future therapeutic applications in neural pathologies such Parkinson's disease, spinal cord injury, and Glaucoma